Quantitative strategies for SR and single molecule dynamics

Development of nano-tools for quantitative bio-imaging

Project description

Over the last ten years, enormous developments in imaging technologies, quantitative analysis algorithms, new optical probes and breakthrough genetic engineering methods have enabled the rapid emergence and application of novel single molecule fluorescence techniques, such as single particle tracking and super-resolution nanoscopy. While these approaches are extremely powerful in Biology, there is an increasing demand for robust, unbiased quantification methods of the raw data. We are developing different analysis algorithms that provide detailed quantification of multicolour single particle tracking data to reveal diffusion patterns and dynamic interactions between individual molecules in living cells. We also develop different analysis algorithms for unbiased quantification of STED and single molecule localization-based super-resolution images.

ICFO groups associated with the project

ICFO publications associated with the project

External collaborations

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Funding

HFSP advanced grant; Fundacio Cellex, MICINN– Plan Nacional MAT2011-22887: IMMU-NANO-FORCE; MINECO-FIS2014-56107-R

Quantitative strategies for SR and single molecule dynamics